With the qiasymphony dsp dna mini kit, in combination with the qiasymphony generead dna ffpe treatment kit and qiasymphony sp, it is possible to fully automate dna extraction from up to 96 ffpe samples. Opposite to that, the ipc was correctly amplified with 6. For a glossary of terms used in this consolidated operating guide, refer to section 11 of the qiasymphony sp as user manual general description. After assay setup, sample lists can be exported to selected realtime pcr cyclers. Documents document view page qiagen qiasymphony spas. The instructions and safety information in the user manual must be followed to ensure safe operation of the instruments and to maintain the instruments in a safe condition. Maintenance procedure for qiasympony sp instrument a weekly preventative maintenance schedule is established for the qiasymphony sp instrument.
Processing challenging forensic casework samples with new. As directly interfaces with the qiasymphony sp, delivering fast, highly reproducible pcr setup. Dsp protocol was able to extract dna from 24 hemagenebuffy coat dna samples in approximately 2 hours with no user interaction beyond initial instrument setup. This flexible and reliable system, based on a readyto. Automated dna extraction from ffpe tissue using a xylene. Print bookmark for use with the qiasymphony spas instruments software version 4. Completevideo qiagen qiasymphony spas dna preparation system qia symphony spas video 3 7729. Extraction of the dna from these samples using manual. The detection limits using the qiasymphony and qiaamp systems were similar 270 and 560 copiesml, respectively. To reduce manual handling steps and minimize the risk of sample contamination, samples processed on the qiasymphony sp can be transferred automatically to the qiasymphony as integrated operation. For instance, after the manual dna extraction, the amplification of the internal pcr control ipc was totally inhibited when concentration of humic acid exceeded 0. A dedicated range of qiasymphony kits increase laboratory efficiency since just a few kits in c. Completevideo qiagen qiasymphony sp as dna preparation system qia symphony spas video 2 7729. The company feels that the adding of qiasymphony sp.
Qiasymphony certal kits, in combination with the qiasymphony sp, enable fully automated purification of nucleic acids from samples generated during production and purification of cellculturebased biotechnologyderived products, biopharmaceutical protein drugs e. Qiagen launches qiasymphony sp device outside the u. For a glossary of terms used in this consolidated operating guide, refer to section 11 of the qiasymphony spas user manual general description. Qiasymphony as module not sold separately as module compatible with various pipetting formats rotorgene discs and 96well plates. Qiasymphony sp as user manual operating the qiasymphony as. Qiagen qiasymphony sp operating manual pdf download. Evaluation of the qiasymphony sp workstation for magnetic. Qiasymphony paxgene blood ccfdna kit handbook preanalytix. View online or download qiagen qiasymphony sp operating manual. Oring test, uvscan and wiping down of conveyor base tray, liquid waste. Use of this product signifies the agreement of any purchaser or user of the qiasymphony paxgene blood rna kit to the following terms. Qiasymphony spas user manual operating the qiasymphony. Compared to the manual dna extraction procedure, the qiasymphony offers several advantages in terms of reproducibility and reliability. Completevideo qiagen qiasymphony spas dna preparation.
The qiasymphony sp performs as well or better than current manual extraction methods without introducing contamination. Qiasymphony workflows samples assays independent operation the qiasymphony as is currently under development, please inquire for availability. Qiasymphony sp manuals and user guides for qiagen qiasymphony sp. Qiasymphony spas user manual operating the qiasymphony sp. Used qiagen qiasymphony spas dna preparation dna related. For use with qiasymphony spas instruments software version 5. We have a total of 2 of these systems left, each one includes qiasymphony sp and as module as well as the stand. A weekly preventative maintenance schedule is established for the qiasymphony sp instrument. Since manual pipetting steps are eliminated, the quality and efficiency of the assays can be ensured even for. We have 1 qiagen qiasymphony sp manual available for free pdf download. The qiasymphony as is a module that directly interfaces with qiasymphony sp. A revised version of chapters 4 of this manual now appears in the. Novel extraction protocols for the qiasymphony sp improve dna yields from forensic casework samples.
Our preliminary results further suggest that the dna recovery of this platform is similar to the one of our manual protocol used for routine stain analyses. Qiasymphony operating software guides the user through setting up a. Qiasymphony as qiasymphony as extends the capabilities of qiasymphony sp to include highly reliable and reproducible automated assay setup. Qia symphony sp, fully automated magneticparticle rna, dna and protein purification for up to 96 samples.
Using a manual plunger operated pipette, the lysate was transferred to 2ml sample tubes sarstedt, numbrecht, germany and loaded onto the qiasymphony sp qiagen. Comparison of qiasymphony automated and qiaamp manual. Detailed instructions for the qiasymphony sp maintenance can be found in the qiagen qiasymphony sp. Management at qiagen is encouraged by the adoption of genereader ngs system as a userfriendly solution for nextgeneration sequencing. Qiasymphony dsp dna mini kit 192, 937236 from qiagen. Qiasymphony technology combines the speed and efficiency of silicabased. Before using the qiasymphony sp as instruments, it is essential that you read this user manual carefully and pay particular attention to the safety information. Used qiagen qiasymphony sp dna related for sale dotmed. Qiasymphony spas general description 122017 9 1 safety information before using the qiasymphony sp as instruments, it is essential that you read this user manual carefully and pay particular attention to the safety information. En qiasymphony spas user manual general description. Blood rna tubes according the manufacturers instructions.
Qiasymphony sp pure performance 012008 ordering information product contents cat. The qiasymphony was able to extract large batches without crosscontamination of samples. Refer to the qiasymphony spas user manual operating the. Mott center 275 east hancock detroit, michigan 48201 fax. Recently we introduced an automated solution for the dna extraction from ffpe tissue using the qiasymphony sp instrument in combination with the qiasymphony dna mini kit. We recommend using elution microtubes cl, racked provided for elution. Qiagen qiasymphony spas dna preparation user manual this is the document view page. Laboratories already operating a qiasymphony sp can easily upgrade their system. Completevideo qiagen qiasymphony spas dna preparation system qia symphony spas video 2 7729. Automated extraction of gdna from buffy coat samples. Qiasymphony spas operating the qiasymphony sp qiagen. For use with qiasymphony sp as instruments software version 5. Qiasymphony a new level in lab automation duration. Descriptions of the tabs, tools, and buttons are listed in separate tables.
So far deparaffinization of the ffpe tissue sections is achieved by manual xyleneethanol pretreatment followed by proteinase k digestion. Before using the qiasymphony spas instruments, it is essential that you read the user manuals carefully and pay particular attention to the safety information. Evaluation of four automated protocols for extraction of. The investigator advanced protocol has a longer heating step that enhances dna binding to magnetic particles, while the high efficiency protocol reduces the loss of purified dna from low elution volumes. Manualslib has more than 27 qiagen manuals checkout popular qiagen categories laboratory equipment manuals. May 2015 qiasymphony sp protocol sheet preanalytix. Detailed instructions for the qiasymphony sp maintenance can be found in the qiagen qiasymphony sp manual. A dedicated range of qiasymphony kits increase laboratory efficiency since just a few kits in combination with optimized protocols cover an extensive range of starting materials and applications. Work lists and rack files may be imported to the instruments, enabling automatic run definition before samples are received in the laboratory. The qiasymphony sp can save you time by processing 196 samples in batches of 24 with sample volumes up to 2 ml. Automated dna extraction using the qiasymphony platform. Yields varied depending on the type and donor of the tissue and ranged from 10. Qiagen kit handbooks and user manuals are available at. Qiasymphony paxgene blood rna kit handbook 032016 5 figure 1.
Mahajan4 march 24, 2010 1this research was partially supported by nsf grants dms9527124, dmi0534862, and dmi0522796, as well as texas atp grant 973604010. Workflow optimization with the qiasymphony system adds. Extracted ccfdna is compatible with different assay applications qpcr, nextgen sequencing, arrays. In the present study, the automated qiasymphony and manual qiaamp extraction systems qiagen, valencia, ca were assessed using paired aliquots derived from clinical wholeblood specimens and an inhouse, realtime pcr assay. Qiasymphony spas user manual operating the qiasymphony as.